What was the source for inspiration?
Global warming and alarming rate of increase of atmospheric temperature is one of the reason for it. whole world is trying to find out alternative source of energy. so, scientists could find ethanol from bamboo.
How this work was done?
Clostridium thermocellum strains SS8 and GS1 grew poorly on crude blopolymers but termented them easily after alkall treatment. With 1% alkall-extracted rice straw (AERS) and dellgnified bamboo pulp (DBP), the ethanol-to-substrate (E/S) ratios were almost the same as those obtained when using fillter paper. Increasing the substrate concentrations decreased the percentage substrate degraded and the E/S ratio and concomitantly increased the amount of reducing sugars accumulated. A maximum amount of 8.6 g ethanol/l was produced by strain SS8 out of 37.5 g DBP degraded. Strain GS1 accumulated reducing sugars at substrate concentrations >50 g/l, thereby accounting for about 70% of AERS degraded. This strain produced cellulase on both cellulose and cellobiose. Both the strains grew in the presence of 1.5% (v/v) ethanol. Strain SS8 fermented starch, but the ethanol yield was low compared to that from cellulose. About 75% of starch degraded accumulated as reducing sugars at a substrate concentration of 40 g/l. The Inhibitory effects of ethanol (2 to 4%) were less drastic when growing cultures were challenged than when they were formed in situ. The effect of ethanol depended upon the phase of the culture.
Who did this great work?
J. M. O. Scurlock
Environmental Sciences Division
Oak Ridge National Laboratory
P.O. Box 2008
Oak Ridge, TN 37831-6407
U.S.A.
D. C. Dayton and B. Hames
National Renewable Energy Laboratory
1617 Cole Boulevard, MS 3311
Golden, CO 80401
U.S.A.
Environmental Sciences Division
Publication No. 4963
Date Published: January 2000
Prepared for the
U.S. DEPARTMENT OF ENERGY
Office of Transportation Technologies
EB 52 03 00 0
and
Office of Utility Technologies
EB 24 04 00 0
Prepared by the
OAK RIDGE NATIONAL LABORATORY
Oak Ridge, Tennessee 37831-6422
managed by
LOCKHEED MARTIN ENERGY RESEARCH CORP.
for the
U.S. DEPARTMENT OF ENERGY
under contract DE-AC05-96OR22464
If you want to know more about this work then you can download this book from here.
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